Journal: bioRxiv

Article Title: Decentralizing cell-free RNA sensing with the use of low-cost cell extracts

doi: 10.1101/2021.05.29.446205

Figure Lengend Snippet: (A) Schematic representation of toehold-mediated RNA sensing. (B) Dynamics of the RNA sensing reactions performed with ZIKV toehold sensor 8 (0.7 nM plasmid DNA) and 27 (2 nM plasmid DNA), regulating the expression of the full-length LacZ in home-made cell extracts and PURExpress cell-free reactions. Error bars represent the standard deviations of three independent experiments, dots are centered at the arithmetic mean for each time point. (C) Example of the endpoint visualization of the experiments after four hours of incubation at 29 °C. (D) Endpoint measurement of RNA sensing reactions performed with ZIKV sensor 27 and trigger 27 in a range of concentrations with and without NASBA isothermal amplification. Gray dots represent data from six independent measurements performed from two independent NASBA amplifications performed on different days. Black error bars correspond to standard deviations of these six measurements.

Article Snippet: Plasmid DNA input was produced by midi prepping an overnight culture of 200 mL LB with the appropriate strain (Promega, A2492) and cleaned again using PCR cleanup (Promega, A6754).

Techniques: Plasmid Preparation, Expressing, Incubation, Amplification